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After a single injection, plasma GH concentrations rose 2- to 10-fold for six days or longer, and IGF-1 concentrations rose roughly 1.5- to 3-fold for nine to eleven days

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Functional assessment of PBMCs and PMNs with Rac1GTP binding assays The pelleted cells are lysed by adding 25 l 2X Radioimmunoprecipitation assay buffer at 4C and centrifuged at 4C in a cold room