The amount of oxidized glutathione (GSSG) can be calculated by subtracting the reduced glutathione (GSH) from the total GSH
It also reduces HbA1c within three months and maintains it thereafter in the diabetic population overall
Oxidative stress is caused by a variety of environmental and dietary factors such as consumption of bad oils, psychological stress, environmental toxins (think mold, air pollution, pesticides), and others
For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)
However, the metabolites in the GSH synthesis, export and reimport pathway decline very slowly, achieving their new steady states in 45 days (data not shown)