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It is believed that stimulation of the brain results in changes in several levels of neurotransmitters, particularly gamma-aminobutyric acid, which can help control seizures

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One hour later, rats were anesthetized with ketamine (90 mg/kg), xylazine (2.8 mg/kg), and acepromazine (0.72 mg/kg) and transcardially perfused with 0.1 M phosphate-buffered saline (PBS) followed by 4% paraformaldehyde (PFA) in PBS