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Cell Culture and in vitro Model of Hemorrhagic Toxicity Astrocytes and 293T cells (Chinese Academy of Sciences, Shanghai) were cultured in dulbeccos modified eagle medium (DMEM) (Gibco, UA) added 10% fetal bovine serum (Gibco, Australia), following which they were incubated at 100% humidity in an environment containing 5% CO 2

After TBST washing (three times, 5 min per wash), the membranes were incubated with the indicated primary antibodies at 4 C overnight with shaking

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Researchers closely examine the results from these trials to see how effective it is in reaching good outcomes