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Substrates were added in a stepwise fashion in the following order (final concentrations) 0.2 mM palmitoyl carnitine, 1 mM malate, 4 mM ADP, 0.4 mM octanoyl carnitine, 5 mM pyruvate, 10 mM glutamate, 10 mM succinate, 1 M CCCP, 2 M rotenone, and 5 M Antimycin A

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Researchers became interested in the combination after observing that glutathione levels tend to decline with age while oxidative stress, inflammation, and mitochondrial dysfunction increase.59 The idea is that providing both cysteine and glycine may help the body rebuild glutathione more efficiently than either nutrient alone

This tripeptide structure is the basis for all biological activities of glutathione

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