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Pulmonary fibrosis studies document reduced interstitial thickness and improved tissue architecture with GHK-Cu treatment

Multivariate statistical analysis was performed using MetaboAnalyst v5.0 (Xia Lab, McGill University, Montreal, Quebec, Canada

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To construct the sorting vector pLKO.1-GST DAF for shRNA gene knockdown experiment, we amplified the coding sequence of N-signal-GST-EGFP-GPI DAF from the pEGFP-GST-GPI DAF plasmid and joined it with FastDigest KpnI/FastDigest BamHI (Thermo Fisher) linearized pLKO.1 vector using ClonExpress II One Step Cloning Kit

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