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Intracellular ROS and lipid peroxidation level Reactive oxygen species (ROS) were measured on the basis of the intracellular peroxide-dependent oxidation of DCFH-DA (S0033S, Beyotime) to form the fluorescent compound 2,7-dichlorofluorescein (DCF)

A 20% infusion of Intralipid in the perfused rat ileum, however, does not significantly increase portal plasma GLP-1 from baseline (Herrmann et al., 1995), suggesting that since orally ingested fatty acids do not reach the ileum, a direct sensing mechanism for this lipid composition does not exist in the ileum or that GLP-1 in this experiment bypasses portal circulation

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Biophysics Mol

(2017) 15:13901396.e1