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Protein bands were visualized using the diamino-benzidine detection kit
On the second day, for all the groups, the different compounds were administered via oral gavage after 1-hour fasting, followed by the choline chloride load the next hour
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Binding induces autophosphorylation of the intracellular kinase domain on tyrosines 1131/1135/1136, recruits substrates IRS-1, IRS-2 and Shc, and activates two major signaling pathways: the PI3K-AKT-mTOR pathway (protein anabolism, cell growth, apoptosis inhibition) and the Ras-Raf-MEK-ERK pathway (cell proliferation, growth gene transcription)
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