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Invert the vial and inject the air into it

The use of Drosophila bring to the study of oxidative liver injury many advantages, including ease of genetic manipulation, availability of genetic mutants and constructs, ease of germ-free generation, relatively short lifespan, amenability to high-throughput screening, and overall cost relative to murine models 40

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J Nutr Biochem 60:4760 Srinivasan MP et al (2021) Differential cytotoxicity, ER/oxidative stress, dysregulated AMPK signaling, and mitochondrial stress by ethanol and its metabolites in human pancreatic acinar cells