We used line-by-line sequential scanning with laser excitation lines 405 for DAPI, 488 nm for Alexa Fluor 488, 561 nm for Alexa Fluor 555 or Cy3, and 633 nm for Alexa 647 or Cy5, with MBS 488/561/633 and MBS -405 beamsplitters
[1] Cell Line:Cultured Sprague-Dawley rat Achilles tendon fibroblasts (passages 2-4) Concentration:2 g/mL Incubation Time:24 h Result:Increased F-actin (polymerized actin fibers, visualized as green fluorescence) levels notably relative to control cells with weak F-actin detection
To establish the true effects of L-C19s chronic fatigue, it is thus necessary to identify and eliminate other common causes of chronic fatigue, such as anemia, hyperglycemia, thyroid disorders, dehydration or diabetes (41)
Ultimately, the best site for you will depend on your personal comfort and routine
10.1111/j.1365-2036.2004.02066.x 105