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The autooxidation of pyrogallol under alkaline conditions generates superoxide, and the SOD-dependent inhibition of this process, tracked by a reduction in the rate of absorbance increase (at 420 nm or 440 nm), constitutes a well-established assay (Marklund and Marklund, 1974)

Improper Storage: Leaving the reconstituted vial out on the bench for hours or trying to freeze it will rapidly degrade the peptide

The peptide is best known for its ability to activate AMPK, a key energy-sensing enzyme that boosts fat metabolism, enhances glucose utilization, and improves mitochondrial function

We used the FindAllMarkers function to identify genes that are differentially expressed between clusters

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