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After incubation with the primary antibodies, the membranes were probed with the corresponding horseradish peroxidase-conjugated secondary antibodies (1:10,000 dilution
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Oncometabolites like succinate, fumarate, and 2-hydroxyglutarate, arising due to mutations in metabolic enzymes like SDH, FH, and IDH, have been linked to changes in DNA and histone modifications, further leading to the activation of EMT and enhancement of cancer stemness properties [28]
The However, these amounts are general estimations
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