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Flow cytometry of human and mouse single-cell suspensions Cells were stained with surface antibodies and Fixable Viability Dye (Zombie UV, BioLegend) in PBS containing 2% fetal calf serum (FCS) (FACS buffer) for 30 min at 4 C in the presence of human (BioLegend) or mouse (BioXcell) Fc block