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When the cellular GSH levels were artificially lowered or raised, TRaQ-G probes reliably detected changes in GSH concentration

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confirmed that TFAP2C regulated GPx1 expression by directly binding to the GPx1 promoter, which contains an AP-2 regulatory region, and the methylation of the CpG island in the GPx1 promoter region prevented the binding of TFAP2C by encompassing the AP-2 regulatory region [81]

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