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After rinsing, slides were incubated with the secondary antibody Alexa Fluor 488 goat anti-rabbit (A-11008 Invitrogen - Thermo Fisher Scientific) or Alexa Fluor 488 donkey anti-goat (A-11055 Invitrogen - Thermo Fisher Scientific) 1:500 in PBS 1 0.5% BSA 0.1% saponin (w/v/v) for 2 h at room temperature
However, in some cases, their symptoms are more extreme, with some requiring hospitalization
This structural complexity is the primary driver behind its elevated market cost compared to older, less sophisticated research compounds