Methods: After collection, the ejaculates of Holstein bulls were subsequently diluted with a Tris extender buffer supplemented with different concentrations of SeNPs (0, 1, 2, and 4 g/ml), followed by semen equilibration at 4C and assessment of sperm viability and motility
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Testing may include SAM, SAH, methionine, homocysteine, whole-blood histamine, copper, zinc, ceruloplasmin, urinary pyrroles, organic acids, lactate, pyruvate, glutathione-related markers, 8-OHdG and lipid peroxides when clinically appropriate