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Western blotting S2 cell pellets were lysed on ice for 30 min in three times packed volume RIPA buffer (50 mM Tris, pH = 8, 150 mM NaCl, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, Pierce Protease Inhibitor Tablet [A32963

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