Gahl WA, Wong-Rieger D, Hivert V, Yang R, Zanello G, Groft S (2021) Essential list of medicinal products for rare diseases: recommendations from the IRDiRC Rare Disease Treatment Access Working Group
Every fourth day (Day 4), cells were split by detaching using Accutase (Sigma, A6964), resuspended in E8 medium supplemented with ROCK inhibitor, and seeded into a new Matrigel-coated surface
As noted above, this is expected and aligns with the protocols design
The conversation also addresses society's approach to handling harmful behaviors, contrasting America's punitive culture with alternative methods of behavioral modification
The safety of physiological MOTS-c does not guarantee the safety of supraphysiological doses