10.3390/ijms21072669 183
Evans blue stain results were measured using a spectrophotometer (Thermo Spectronic Genesys 10 UV, Thermo Fischer Scientific Inc., Waltham, MA, United States) at 610 nm and quantified with a standard curve ( Western Blot Analysis Western blot analyses were performed as previously reported ( n = 6 per group)
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It also has been found to exert antitumor activity by inhibiting cell proliferation, angiogenesis, inducing cell apoptosis, and autophagy
Bacterial cell death was measured through incubation with propidium iodide (Pi) which cannot be taken up by viable bacteria