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The quantitative analysis of each sample was performed by Exion ultra-high performance liquid chromatography (UPLC) coupled with a triple quadrupole mass spectrometer (SCIEX, QTRAP 6500+), equipped with electrospray IonDrive Turbo-V Source

GSH and GSSG were extracted using perchloric acid (HClO 4 ) solution from the homogenized fruits

The Metabolic Window: Why Fasted-State Dosing Changes Everything 5-Amino-1MQ inhibits NNMT, an enzyme concentrated in adipose tissue that methylates nicotinamide (a precursor to NAD+) and removes it from the NAD+ salvage pathway

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Antioxidant activity assays The antioxidant capacity of CM-EVs was evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging assays, with results expressed as Vitamin C equivalents (mol/L)