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79 Although liver biopsy is considered as the gold standard, its acceptance rate is relatively low due to various limitations

Storage is not just a technical instruction

Cell culture studies HEK293T cells (CRL-3216, ATCC) were cultured in DMEM (11995073, Life Technologies) supplemented with 10% heat-inactivated FBS (10500064, Life Technologies), 100 IU ml 1 penicillin and 100 g ml 1 streptomycin solution (Pen/Strep, P4333, Sigma Aldrich)

Nikolova MP, Kumar EM, Chavali MS

These experiments have been successfully repeated using NPC overexpressing Nrf2, however, the number of NPC needed to show similar protection was 5-times less than the primary astrocytes unpublished data