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He wound up living on the streets, and at the age of twenty-one he was convicted on charges of drug possession and incarcerated

To avoid interferences due to different expression levels of GFP-constructs among cell population, mean fluorescence intensity was measured at the GM130- or MannII-BFP-positive Golgi region as well as at the total cell area, and ratio values were used to determine the relative Golgi accumulation of the constructs

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