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This difference has a direct consequence for how the protocol is structured

These findings highlight the important role of the carnitine pool as a bridge between cellular metabolism and epigenetic regulation and open the possibility of developing novel therapeutic strategies targeting specific metabolic and epigenetic targets

(2009) 296:C125870

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At 12 hpi, cells were lysed by resuspending in 3 mL of mild lysis buffer (10 mM Tris, pH 7.4, 150 mM NaCl, 2 mM MgCl 2 1 mM EDTA, 1 mM DDT and 0.5 % Igepal) and incubated on ice for 10 min, followed by vortexing for 30 s