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After deparaffinization, samples were stained with the HE solution (FUJIFILM Wako Chemicals Corporation, Osaka, Japan) or immunostained for 4-HNE (antigen retrieval treatment), F4/80 (no antigen retrieval treatment) as follows

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The slides were observed under a microscope, and images were captured and analyzed

Its moments like that where youre like, Whoa, whoa, whoa