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In human clinical trials, a marked increase in glutathione levels was shown regardless of its initial (basal) concentration

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The OD 600 was measured every 15 min during continuous linear shaking (1096 cycles min 1 , 1 mm orbit) at 30 C for 24 h

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Exclusion criteria were a history of bariatric surgery, alcohol use disorder identification test (AUDIT) greater than 16, hemoglobin A1c measurement greater than 9, a history of seizure disorder or traumatic brain injury, on specific medications that interfere with alcohol metabolism such as certain antibiotics (reviewed on a case-by-case basis by study physician), or currently pregnant or lactating