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83 The folate cycle uses glycine and tetrahydrofolate (converted from folic acid) and produces intermediates (5,10-methylene-tetrahydrofolate and 5-methylene-tetrahydrofolate) to supply methyl groups for purine synthesis

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For the quantitative evaluation of the results of Y2H, the transformed single colony with the same size on SD-L/W medium was transferred to the 10 mL liquid SD-L/W/H/A medium, and the OD 600 values were determined after shaking culture for 24 h

Cloudiness or unusual particulates suggests poor reconstitution, contamination, or a quality issue