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The mitochondrial pellet was resuspended in MSHE buffer with BSA and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific)

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Berthelot L, Papista C, Maciel TT, Biarnes-Pelicot M, Tissandie E, Wang PH, et al

Research shows it can sequester reactive oxygen species outside cells while uncoupling electron transport chains in liver mitochondria to lower intracellular oxidative stress production [19]