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Cells were washed three times with PBS and incubated with a donkey anti-mouse Alexa Fluor 568 (ThermoFisher Scientific, A10037) and donkey anti-rabbit Alexa Fluor 488 (ThermoFisher Scientific, A21206) secondary antibodies (1:400) for one hour in the dark

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Transcription factor Nrf2 coordinately regulates a group of oxidative stress-inducible genes in macrophages