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Supernatants were collected, and the amount of glycerol was quantified spectrophotometrically at 540 nm with the free glycerol reagent colorimetric kit
Although not applicable for GLP-1, fusion to the C-terminal end of albumin could be an attractive approach to avoid cleavage by CPA
smegmatis does not export GS [23], it may be that, similar to M
Thus, the activation of Ras protein 1 and phospholipase C, as well as the activation of the IP3 and diacylglycerol (DAG) pathways (15), induces calcium ion inflow, triggers the exocytosis of insulin particles, and results in insulin secretion (15, 51)