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A deficiency in one nutrient can increase the likelihood of a deficiency its all connected

All animals were kept and the experiments were performed in accordance with the European Community guidelines for the use of experimental animals (86/609/EEC)

T.et al (2011)

On the experiment day, all subjects consumed the same breakfast (200 g of steamed rice) 90 min before the exercise session to normalize the effects of a pre-exercise meal

Thereafter, blood samples were obtained through the cardiac puncture into EDTA coated vials and serum samples were collected for biochemical analysis as per our previously published protocol 33