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This solvent was composed of 40 L of 50 mM NEM (Sigma-Aldrich) in a mixture of 80% methanol and 20% 10 mM ammonium formate (Sigma-Aldrich), combined with 40 L of 100% acetonitrile (Thermo Fisher)

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[1] [3] Decisions about whether to continue or temporarily pause these medications are individualised based on factors such as: The specific GLP-1 medication and dosage Whether you're in a dose-escalation phase Presence of gastrointestinal symptoms Type and urgency of surgery Individual risk factors for aspiration Standard UK fasting guidance typically recommends 6 hours for solids and 2 hours for clear fluids before surgery

178 Triptolide also demonstrated multiple beneficial effects in IBS models, including reduction of colonic inflammation and modulation of gut microbiota composition