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Leaves samples (100 mg) were homogenized with 1 ml of an ice-cold extraction buffer containing 50 mM potassium phosphate buffer (pH 7.0), 2 mM 2-mercaptoethanol, 0.1 mM EDTA, 0.5% (v/v) Triton X-100, 2% (w/v) polyvinylpyrrolidone, and 1 mM phenylmethylsulfonyl fluoride (PMSF)

The 12 mg group achieved 23.7% average weight loss (27.2 kg or 60 pounds), while the 9 mg group lost 20.0%

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