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In vitro experiments on lung cancer cells revealed that L-asparaginase treatment significantly induced autophagy activation in A549 cells, leading to their apoptosis (108)

Proper spacing of doses and selecting alternative antibiotics when appropriate can mitigate this risk

Likewise, to avoid unpleasant side effects of systemic carbonic anhydrase inhibition, carbonic anhydrase inhibitors, since acetazolamide (topical administration with little or no effect on intraocular pressure) [68,69], were used for a long period before a topical carbonic anhydrase inhibitor was realized (dorzolamide) [70,71]

Many experts suggest that a "dual-action" approach is most effective

Subsequent work established that the peptide was naturally complexed with copper in plasma and that both the peptide and the copper were necessary for its biological effects