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Afterwards, NP40 alternative was added to a final concentration of 0.4% ( v / v ), and cells were lysed by five consecutive freeze/thaw cycles

A decrease in the CD18 expression led to a decrease in the inflammatory process, indicating that zerumbone has the potential to be developed into an immunosuppressant ( + and CD4 + in splenocytes (Jantan et al., 2019)

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All the reactions were operated under a nitrogen atmosphere using a Schlenk-line system and a glovebox to protect air- and moisture-sensitive chemicals