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The collected pellets were resuspended in 60 L of PBS and 15 L of 5 SDS-PAGE sample buffer, and the mixtures were boiled for 10 min to denature the proteins
However, the disappearance of GSH was not accounted for by its conversion to GSSG as this form was not detected in the representative HPLC chromatogram shown
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Interestingly, excessive ROS production and oxidative stress raises the binding affinity of the spike protein for the human ACE2 receptor (Hati and Bhattacharyya, 2020
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