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Activation of N -methyl-d-aspartate receptor regulates insulin sensitivity and lipid metabolism

Mitochondrial dysfunction is induced by high levels of glucose and free fatty acids in 3T3-L1 adipocytes

Sterile water provides a preservative-free option but offers no antimicrobial protection

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A colourless or green-tinged solution indicates the complex has been disrupted rather than simply diluted, and visible absorption spectrophotometry around 500-600 nm is the standard bench check on complex integrity