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We further disclosed the effect of TME hypoxic condition on ROS generation by dispersing NaBi V O 3 -PEG in degassed oxygen-free PBS that was filled with nitrogen and then examining signal intensities

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By removing the need for centrifugation, filtration, or column equilibration, processing times are significantly shortened

Cells treated with test compounds were washed with PBS and incubated with 10 M 2,7-dichlorofluorescein diacetate (DCFH-DA) at 37 C for 30 min, followed by washing with PBS, digestion with trypsin, and collection