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After BALF collection, the pellet was immediately incubated with 1x Ammonium-Chloride-Potassium (ACK) buffer (150 mM NH 4 Cl (Sigma-Aldrich), 10 mM KHCO 3 (Sigma-Aldrich), 0.1 mM Na 2 EDTA (Sigma-Aldrich), pH 7.4) for 5 min at room temperature and cells were washed twice with 1x PBS

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