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Images were then acquired using an inverted microscope

To assess that these cells form monolayer on the membrane inserts, transepithelial resistance was routinely monitored using an epithelial volt-ohm-meter including STX-2 electrodes (EVOM, World Precision Instruments, Berlin, Germany)

albicans can sense amino acids through the Ssy1p-Ptr3p-Ssy5p (SPS) sensor and hydrolyze activated transcription factors Stp1 and Stp2 to utilize amino acids as a carbon source [20,21,22,23]