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It was previously shown that BDNF has anti-inflammatory effects that counteract various inflammatory cytokines (Chen et al., 2013

LC-MS confirms identity by measuring the molecular mass and comparing it to the theoretical value (403404 g/mol for the parent complex, 462 g/mol for the acetate salt)

Some experience mild side effects, such as slight redness at the injection site, but these typically fade quickly

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Subsequently, 0.4 mL of a PI staining/lysis solution (0.5% NP-40, 0.5 mM ethylenediaminetetraacetic acid [EDTA]) and 50 L of RNase A (10 mg/mL in TrisEDTA buffer, pH 8.0) solution were added