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However, the reduction in colony formation upon combined RSL3-treatment and LDHB silencing was only slightly rescued by GSH-mee supplementation (Fig

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Strikingly, the core features of oxytosis , including inhibition of the cystine/glutamate antiporter (system xc ), GSH loss, iron-dependent lipid peroxidation, and mitochondrial dysfunction, align precisely with the hallmarks of ferroptosis defined over a decade later (Dixon et al., 2012)

If tolerating well after 1-2 weeks with no adverse effects, you can increase to the standard protocol