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The supernatants were transferred to autosampler vials and analyzed for heroin, morphine, and morphine-3-D-glucuronide (M3G) using an Acquity UPLC system (Waters, Milford, MA) coupled to a Xevo-TQS triple quadrupole mass spectrometer with an electrospray ionization interface (Waters) based on a method previously described 12

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Colozza G, Koo B