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SIRT3 activity Protein was extracted from cells and tissue using a mild lysis buffer (50 mM Tris-HCl pH 8, 125 mM NaCl, 1 mM DTT, 5 mM MgCl 2 , 1 mM EDTA, 10% glycerol, and 0.1% NP-40)

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Laboratory models analyze MOTS-c for its impact on bone density signaling and its role in promoting bone-repair-associated gene expression

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Insert the needle through the center of the rubber stopper of the bacteriostatic water vial

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