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In GIPR, Q138 1.40b and two glutamic acid residues (E135 1.37b and E288 45.52b ) make three hydrogen bonds with Y10 P and T7 P , while F22 P and MeL13 P /L14 P in retatrutide interact with Y36 ECD and R131 1.33b through stacking and hydrophobic contacts, respectively

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Inert hardware and coated vials are essential to reduce adsorption and improve reproducibility Controlling these factors is mandatory for producing a robust and stability-indicating LC method

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