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Cell viability assay For cell viability assay, cells were planted in 96-well plates (34 10 3 per well) after indicated treatment, then incubated with 10% CCK-8 reagent at 37 C for 2 h

TNF- also synergizes with IFN- to simulate the production of reactive nitrogen intermediates for mycobacterial killing within macrophages ( Our preclinical studies confirmed the previous findings that the T2DM group has lower levels of IFN- ( Figure 3E ) and TNF- ( Figure 3F ) when compared to healthy individuals

Glutathione is a naturally occurring antioxidant found in every cell of your body