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Cells were dehydrated by subjecting them to ascending series of alcohol 50%, 70%, 80%, 90% and 100% followed by treatment with Epoxy resin for 24 h

Thus increasing ROS levels are not conducive to embryo growth and result in impaired development

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13 C NMR (100 MHz, Chloroform-d) = 163.0, 162.5, 149.3, 146.8, 138.4, 125.7, 124.7, 115.0, 114.8, 65.6, 59.4, 32.0

The non-exposed mice were taken directly from their home cage in the housing room for perfusion