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500 lL from each sample was transferred to tubes containing 500 ice-cold acetonitrile with internal standard per incubation sample

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One of the commonly reported microbiome differences between obese/HFD-fed animals and lean controls is an increased Firmicutes : Bacteroidetes ratio 43,44

TB-500 is dosed less frequently, typically once or twice per week during a loading phase and once every two weeks during maintenance, reflecting its longer half-life and systemic distribution pattern

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