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Briefly, flash-frozen liver was homogenized in ATP assay buffer using Dounce homogenizer

OsDHAR catalyses the GSH-dependent reduction of DHA to recycle AsA in a two-step reaction: the first step is the cysteine oxidation reaction and second step is the cysteine reduction reaction

Ward PD, Miller HL, Shipman AR

Under physiological or untreated pathological conditions, mitophagy functions beneficially by maintaining mitochondrial integrity, reducing ROS accumulation, suppressing oxidative stress, and consequently decreasing angiogenesis and lesion viability in EESCs

Consequently, EESCs secrete angiogenic factors in a paracrine fashion, thereby fueling neovascularization (29) and further advancing endometriosis pathogenesis