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The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH 2 O and PBS, and then probed with the primary antibody (EM1901-56) at 1/2,000 dilution for 1 hour at room temperature

For patients who do weekly dosing, they should consider not taking their dose at least seven days before the surgery

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Reduced autophagic flux was also reflected by the partial accumulation of the autophagy adaptor and substrate p62, taken as a surrogate for autophagic flux, in TBC1D9B-depleted cells (Supplementary Fig

Reduced GABAergic activity leads to an increased likelihood of neural excitability and seizure development