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HepG2 cells were cultured in Dulbeccos modified Eagles medium (DMEM) with 1% penicillin-streptomycin and 10% fetal bovine serum (FBS), incubated in a standard humidified incubator

For the PM group, on day 0, the tumor cells were injected as in the M group

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This case emphasizes the importance of considering vitamin B12 deficiency as a reversible cause of hemolysis

Methyl Life specializes in methylcobalamin B12 shots, which are ideal for individuals with MTHFR gene mutations